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土壤微生物基因组DNA抽提实验说明
发布时间:2017-06-07 点击: 次   编辑:admin

kpl竞猜平台 (HAIKEYITIQURUCHENJIWU,PAIXIEWU,FEISHUI,XUESHUIDENGYANGPINDEDNA)TURANGWEISHENGWUJIYINZUDNACHOUTISHIJIHE/TIQUSHIJIHE

(LIYONGGUIXIFUDNAYUANLI)

一,说明

kpl竞猜平台 BENSHIJIHEKEYIZAI30FENZHONGNEIKUAISUYOUXIAODEZHIJIECONGTURANGFENLIWEISHENGWUJIYINZUDNA。YONGSHANGHAIJINGXINKEJI(QUANZIDONGYANGPINKUAISUYUNJIANGJI)FSTPRPYIQI(JIANGYANGPINYUHUANCHONGYEJIARUDAIYOUTESHUXIAOZHUDEYANGPINGUAN/LIXINGUAN)ZAI40MIAONEIKEYILIEJIETURANGZHONGDEZHIWU、DONGWUZUZHI、XIJUN、ZAOLEI、ZHENJUNBAOZI、JIAOMU、XIANCHONG。JINGGUOBENSHIJIHEDEFENLICHUNHUA,SUODEDEDNAKEYIYONGYUPCR、XIANZHIXINGMEIQIE、DIANYONGHEQITASHIYONG。

kpl竞猜平台 NONGSUOSEWS-MSHIYONGQIANJIARU25MLWUSHUIYICHUN,BINGZAIPINGZISHANGZUOHAOBIAOJI,GAIHAOPINGGAI

WASH DSHIYONGQIANJIARU56MLWUSHUIYICHUN,BINGZAIPINGZISHANGZUOHAOBIAOJI,GAIHAOPINGGAI

kpl竞猜平台 YONG1ML  ddH20(MIEJUN)WANQUANRONGJIERNAMEI,JIANGRNAMEIFANGRUSHUIZHONGZHU,SHUIFEIHOUZAIZHUFEI20FENZHONG,BINGDIAOYAN,HUANMANLENGZHISHIWENHOU,JIANGRNAMEIQUCHU,QUANBUJIARU sodium phosphate bufferZHONG,CHONGFENHUNYUNHOU,JIANGsodium phosphate buffer buffer FANGRU4DUBAOCUN。

SHIJIHEZUCHENG

二,步骤

JIARU500MG TURANGYANGPINDAOYIGEYANGPINGUANZHONG,GUANZHONGYINGGUANYOU0.05-0.1ml  KONGJIAN。

(ZHUYIYI,WEISHIYANGPINJINKENENGYAODUOJUNYUN,YIGEYANGPINYIBANQUSIGEGUAN,LIANGGUANBINGYIGUAN,DEDAOLIANGGUANDEDNA,ZHUYIER,ZAISHIYANZHONG,KEYONGPUTONGLIXINGUAN+YANGHUAGAOZHU(0.8g),ZHUYISAN,YINGJIANGFANGZAI-20DUDEYANGPINSHANGYITIANQUCHUHUADONG)

JIARU0.1956ml  sodium phosphate buffer DAOYANGPINZHONG

JIARU0.0244ml MT buffer DAOYANGPINZHONG

YONGSHANGHAIJINGXINKEJI(QUANZIDONGYANGPINKUAISUYANMOJIJI)YIQIYISUDU14DANWEIPINZHEN60S FANFUSANCI(QUEBAOWANQUANKEYIFENSUI)

13000rmp  LIXIN5-10min ,CHENDIANSUIPIAN    (ZHUYIYI,JIAYIBUQUCHUFUSUANDEBUZHOU:QUSHANGQINGDAO10YANMODANWEILIXINGUAN,JIARU。。。。。。。。。。。。。DIANDAOHUNYUN,2MIN   12005MINKANBUQING)

kpl竞猜平台 JIANGSHANGQING(YUE0.12ml)ZHUANYIDAOYIGEGANJINGDE2YANMODANWEILIXINGUANZHONG,JIARU0.05ml  PPSDAOGUANZHONG,YONGSHOUDIANDAOHUNYUN10CI。

13000RPM LIXIN5MIN YICHENDIANDANBAIZHI,ZHUANYISHANGQINGDAOYIGEGANJINGDE2YANMODANWEILIXINGUANZHONG(YUE0.14ml)

kpl竞猜平台 JIARU1ML Binding solution b(5BEITIJI)DAO2YANMODANWEILIXINGUANZHONG,YONGSHOUDIANDAOHUNYUN2MIN (TIQIANSHUIJIARE,CUJINDNAYUGUIJIAOJIHE)

kpl竞猜平台 JIANGJIEQUANZHUFANGRU2MLSHOUJIGUANZHONG,JINGHUNHEYEJIARUJIEHEZHUZHONG,SHIWENFANGZHI2 min.(FENSANCIJIA,MEICIJIA0.12-0.14mlLIANGGUANBINGYIGUAN)

kpl竞猜平台 13000RPMLIXIN1MIN,DAODIAOSHOUJIGUANZHONGDEFEIYE,JIANGJIEHEZHUZHONGXINFANGRU2YANMODANWEILIXINGUANZHONG。

kpl竞猜平台 JIARU0.1ml SEWS-M(Spin-buffer)DAOJIEHEZHUZHONG,13000RPMLIXIN1MIN,DAODIAOSHOUJIGUANZHONGDEFEIYE。

ZHUQUZHUFUZHISUAN

kpl竞猜平台 ZAI1.5YANMODANWEILIXINGUANZHONGZHIBEIFUZHISUANXIDIRONGYE:

Sodium phosphate buffer  978UL

kpl竞猜平台 MT  buffer            0.0244ml

kpl竞猜平台 Pps:                  250ul

HUNYUN,13000RPMLIXIN1FENZHONGZHUANYISHANGQINGYEYUYIGEGANJINGDE2YANMODANWEILIXINGUANZHONG。

kpl竞猜平台 JIARUDENGTIJIDE5M Guanidine Thiocyanate sojution HUNYUN。

JIARU0.1ml FUZHISUANXIDIYEDAOBUJU11ZHONGDEJIEHESHUZHONG,13000RPMLIXIN1FENZHONG,DAODIAOSHOUJIGUANZHONGFEIYE。

kpl竞猜平台 ZHONGFUSHANGSHUBUZHOUSANCI

JIEBUZHOUSHIER

JIARU0.13mlwashD  YUJIEHEZHUZHONG,13000RPMLIXIN1MIN,DAODIAOSHOUJIGUANZHONGDEFEIYE。

JIANGJIEHEZHUZHUANGRULIXINGUANZHONG,130000RMPLIXIN2MIN

JIANGJIEHEZHUZHUANGRU1.5YANMODANWEILIXINGUANZHONG。SHIWENFANGZHIWUFENZHONG,YIBIANYICHUNHUIFAGANJING,ZAIMOZHONGYANGXIAOXINJIARU,0.015ml DES  。BUYAOCHUOPOMO,SHIWENFANGZHI2MIN。(37DU  30MIN)

13000RPMLIXIN1MIN LIXINGUANZHONGJIWEIFENLIDEDNA,ZHUCUNYU-20DUHUOJINRUXIAYIBUSHISHIYAN。(YINGJIANG2GUANDNAHUNYUYIGUAN,HUNYUN,ZAIFENCHENGLIANGGUAN,ZHEIYANGJINENGDEDAO4GUANYIGUANDEMUDE。MUDESHIJINLIANGXIAOCHUYANGPINNEIBUCHAYI)

BENSHIYANBUYONGQUCHUFUZHISUANZHEIYIBU,DANYOUWENXIANZHICHU,ZAI-20DUSHI,FUZHISUANRENGHUIJIANGJIEDNA,

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