kpl竞猜平台

所在位置:kpl竞猜平台 > 新闻动态 > 技术知识 >

技术知识
联系方式
地址:上海市闵行区七莘路1839号财富108广场南楼2510室
电话:021-57790908
传真:021-54298177
网站:zxkky.com
简便快捷地提取真菌DNA的2种方法
发布时间:2017-05-24 点击: 次   编辑:admin

实验目的

DUIZHENJUNXIBAOJINXINGPOBI,TIQUQIDNA。

实验原理

SUIZHEFENZISHENGWUXUEJISHUDEJINZHAN,PCRXIANGGUANFANGFAYUELAIYUEDUODIBEIYINGYONGDAOZHENJUNXUEYANJIUZHONGLAI,ERZHENJUNDNADETIQUSHIBAOKUOFENZIZHENDUANZAINEIDESUOYOUSHIYANDEJICHU,JIANBIANKUAIJIEDITIQUZHENJUNDNA,QIEHUODEDEDNAWANZHENG、CHUNDUGAO,DUIZHENJUNFENZISHENGWUXUEDEYANJIUYOUJIQIZHONGYAODEYIYI。

ZHENJUNXIBAOBIYIJIDINGZHI、PUTAOTANGWEIZHUGOUCHENGXIBAOBIGUJIA,ERJIZHIZEYOUDUOTANG、GANLUJUTANG、TANGDANBAI、ZHIZHIDENGTIANCHONG。SIZHUANGZHENJUNJIANRENHOUBIDEGOUCHENG,XUYAOJIAOWEIFANSUODEPOBISHOUDUAN,GUZHENJUNDNADETIQUJIAOXIJUNHUOQITAZUZHIXIBAONANDUGENGDA。WEIBAOZHENGZHENJUNPCRJISHUJIQIXIANGGUANFENZISHENGWUXUEJISHUDESHUNLIKAIZHAN,JIANBIANKUAIJIEDITIQUZHENJUNDNA,QIEHUODEDEDNAWANZHENG、CHUNDUGAO,SHIBIXUJIEJUEDEQIANTITIAOJIAN。

实验材料和器具

YANGPIN:ZHENJUNLEI

YIQI:GAOTONGLIANGZUZHIYANMOYI(SHANGHAIJINGXIN,Tissuelyser-48)

HAOCAI:LIXINGUAN(SHANGHAIJINGXIN,0.4YANMODANWEI),YANMOZHU(SHANGHAIJINGXIN,6HAO,1KE)

SHIJI:TE Buffer,DA Buffer,E Binding Buffer,Elution Buffer

实验步骤

FANGFA1

1.1 QU0.1gJUNSIJIARU0.4YANMODANWEILIXINGUANZHONG,JIA0.08mlTE Buffer,JIARUSHIYINGSHA100mg;

1.2 JIANGLIXINGUANFANGRUSHANGHAIJINGXINKEJIDEGAOTONGLIANGZUZHIYANMOYITissuelyser-48ZHONG,SHEZHIPINLV13DANWEIZHENPIN,YANMO10min;

1.3 QUCHULIXINGUANYU65°CHUANJINGZHONGWENYU30min,RANHOUYICHU;

1.4 JIARU0.65mlDA Buffer,HUNHEJUNYUNHOUYUBINGYUZHONGFANGZHI5min;

kpl竞猜平台 1.5 FANGRULIXINJI14000r/minLIXIN3min;

kpl竞猜平台 1.6 JIANGSHANGQINGYEZHUANYIDAOYIGEXINDE0.5YANMODANWEILIXINGUANZHONG,JIA0.15mlDEE Binding Buffer,BINGHUNHEJUNYUN,JIANGHUNHEYETIZHUANYIZHISpin column;

kpl竞猜平台 1.7 FANGRULIXINJI6000r/minLIXIN1min,QIQUJIEYEGUANZHONGYETI;

1.8 Spin columnZHONGJIARU0.12mlDEWash Buffer,YU10000r/minLIXIN30s,QIQUJIEYEGUANZHONGYETI;

1.9 ZHONGFUBUZHOU1.8,QIQUJIEYEGUANZHONGYETIHOU,ZAI10000r/minLIXIN60s,Spin columnZHUANDAOXINDEEPGUANZHONG;

1.10 Spin columnZHONGJIARU0.03ml Elution Buffer,SHIWENFANGZHI1min。12000 r/minLIXIN60s,BINGQIQUSpin column。

FANGFA2

kpl竞猜平台 2.1 QU0.1gJUNSIJIARU0.4YANMODANWEILIXINGUANZHONG,JIA0.08ml10×TE Buffer,JIARUSHIYINGSHA100mg;

2.2 JIANGLIXINGUANFANGRUSHANGHAIJINGXINKEJIDEGAOTONGLIANGZUZHIYANMOYITissuelyser-48ZHONG,SHEZHIPINLV60hz,YANMO2-3min;

2.3 JIARU0.024ml10%SDS,JIARU0.002mlDANBAIMEIK,HUNYUN,65°CHUANJINGZHONGSHUIYU30min;

2.4 RANHOUWOXUAN1min,JIARU0.024ml5M NaCl,JIARU1/10TIJIDE10%CTAB(YUE0.013ml);2.5 YU65°CSHUIYU60min,WOXUAN1min;

2.6 CHOUTI,JIARU1/2TIJI(YUE0.07mi)DETrisBAOHEFENJI1/2TIJI(YUE0.07ml)DELVFANG:YIWUCHUN(24:1),HUNYUN;

2.7 YULIXINJI14000ZHUANLIXIN1min,QUSHANGQINGYEDAOLINGYILIXINGUANZHONG;

2.8 ZHONGFUBUZHOU2.7 2-3CI(SHILIANGXIANGJIEMIANZAZHIDUOSHAOERDING)。

2.9 JIARUDENGTIJILVFANG:YIWUCHUN(24:1)HUNYUN,14000ZHUANLIXIN10min,QUSHANGQINGYEZHILINGYILIXINGUANZHONG;

kpl竞猜平台 2.10 ZUIHOUKAISHICHENDIAN,JIARU0.045ml NH4Ac,QINGROUHUNYUN,BINGSHUIZHONGFANGZHI30min,JIARU0.6BEITIJIDEYIBINGCHUN,HUNYUN,14000ZHUANLIXIN10min,QISHANGQINGYE;

2.11 YONG1000ul70%YICHUNQINGXICHENDIAN,SHIWENLIANGGAN,JIA0.04ml TE BufferRONGJIECHENDIAN。

实验结果

真菌实验

上海净信实业发展有限公司提供专业均质器无菌均质器冷冻研磨仪、等实验室仪器设备。      沪ICP备14048887号-3    电话:021-57790908  传真:021-54298177      网站地图