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高通量组织研磨仪研磨果蝇的法和提取DNA的实验步骤
发布时间:2017-05-22 点击: 次   编辑:admin

实验目的

kpl竞猜平台 DUIGUOYINGJINXINGYANMO,TIQUQIRNA。

实验原理

kpl竞猜平台 TONGGUOYANMOZHUDEZHUANGJI,CHONGFENYANMOGUOYING,JINERTIQUQIRNA。

实验材料和器具

YANGPIN:GUOYING

kpl竞猜平台 YIQI:GAOTONGLIANGZUZHIYANMOYI(SHANGHAIJINGXIN,Tissuelyser-24)

HAOCAI:LIXINGUAN,BUXIUGANGYANMOZHU(SHANGHAIJINGXIN,6HAO)

实验步骤

1.QU5-10ZHIGUOYINGFANGRUYANMOGUANZHONG,GAIJINYANMOGUAN. JIARUYANMOZHU,13DANWEIZHENPIN,1min,CHONGFENYUNJIANG(SHIJIANKEGENJUYANGPINBENSHENDENANYICHENGDULAIDIAOJIE,NANMODEYANGPIN,KEYIYANZHANGSHIJIAN,RUYICIYUNDONGLIANGFENZHONGHAIBUSHIHENCHONGFEN,KEYIJIANXIDEZHONGFUGONGZUOJICI)

2.JIANGYANMOGUANFANGRUGAOTONGLIANGZUZHIYANMOYIZHONG,60M/SYANMO30S

3.JIQITINGZHIHOU,QUCHUYANMOGUAN,FANGRULENGDONGLIXINJIZHONG,YU4DU,10000gLIXIN10FENZHONG

4.XIAOXINXIQUSHANGQINGYE0.1YANMODANWEIZHIXINDE0.3YANMODANWEILIXINGUANZHONG,GAISHANGGUANGAI,ZAISHIWENSHANGFUYU15FENZHONG,SHIYANGPINCHONGFENLIEJIEZHIYUNJIANGYEJIAOTOUMING,RUGUORENGYOUSHAOLIANGKELISHUYUZHENGCHANGQINGKUANG,BUYINGXIANGRNATIQUZHILIANGHEDELV。

5.ZAI0.3MLLIXINGUANZHONGJIARU0.02YANMODANWEILVFANG,GAIJINGUANGAI,ZHENDANGHUNYUNBINGJIANGQIZAIBINGSHANGFUYU15FENZHONG,YU4DU,12000gLIXIN15FENZHONG。LIXINHOUHUNHEWUFENCENG:SHANGQINGCENG,ZHONGJIANCENG,XIACENG;RNACUNZAIYUSHANGQINGCENGZHONG。

6.JIANGSHANGQINGCENGXIAOXINZHUANYIDAOYIGANJINGDELIXINGUANZHONG,JIARUDENGTIJIBINGYUDEYIBINGCHUN,DIANDAOZHENDANGHUNYUN,JIANGHUNHEDEYANGPINZAI-20DUTIAOJIAN,FUYU20FENZHONG,YU4DU,12000gLIXIN10FENZHONG。RNACHENDIANTONGCHANGXINGCHENGPIANZHUANGCHENDIANFUZHEYUGUANBIHUOGUANDI。

7.XIAOXINQIQUSHANGQING,YONG0.2TIJIDEBINGYU75%DEYICHUNXIDIRNACHENDIANYICI,DIANDAOXIDILIXINGUANGUANBI,BINGXUANWOZHENDANGYANGPIN,JINKENENGRANGCHENXUANFU,YU4DU,12000gLIXIN5FENZHONG,ZAICIQUCHUSHANGQING,LIANGGANCHENDIAN。

kpl竞猜平台 8.CAOZUODEZUIHOU,ZAIYINLIANGCHUSHIDUGANZAORNACHENDIAN(BIMIANGUOFENGANZAO,NEIYANGHUIJIANGDITADEKERONGXING)。

9.YONGSHILIANG(YIBANKEYONG0.01—0.02YANMODANWEI)DEWURNAMEISHUIHUOTERONGYELAIRONGJIERNA。CHOUTIHAODERNA,BAOCUNYU-70DU。

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